One of the most fascinating observations and one in all the best in vitro experiments that signifies these opposite effects of sterols and fatty acids is their affect upon the oxidation processes by which red cells intervene. When a sample of odd venous blood is treated with a preparation of cholesterol or nonsaponifiable fractions, using the above mentioned technique and after its separation from the cholesterol it is agitated with air or oxygen which is handed by these samples, the colour turns into a vivid vermilion crimson, and this persists for a long time. When the identical venous blood is treated with a preparation of polyunsaturated fatty acids as mentioned above, the color becomes very darkish, virtually black purple. When air or oxygen is handed through these samples, the blood becomes lighter in colour for less than a short while, the darker color reappearing within a couple of minutes. One is instantly impressed by the similarity of the cholesterol treated blood to arterial blood, whereas the fatty acid handled blood is much like venous blood, and especially to the shade of venous blood in instances of shock.

We tried to tie in these findings with the remark of Binet regarding the modifications in blood fatty acids when passing by way of the lungs. He has been ready to show that the amount of the polyunsaturated members seems to be decreased by the passage of blood by way of the lungs. We may show that the purple cells leaving the pulmonary vascular mattress are somewhat richer in the unbound cholesterol than they've been within the blood which entered the lungs. The lipid content material is altered in an opposite approach because the blood travels by means of the final circulation. That's, the polyunsaturated fatty acid content is increased in the pink cells while the quantity of free cholesterol appears to be diminished. The sterol richer crimson cells appear capable of retaining for an extended period of time, the quantity of oxygen which hemoglobin has fixed, while a speedy reduction of oxyhemoglobin is seen in the pink cells when the polyunsaturated fatty acids intervene.

This led us to think about an intervention of these two teams of lipids in relation to the oxygen transportation by the pink cells. Bearing in mind the truth that whereas cholesterol reduces cell permeability and polyunsaturated fatty acids improve it, an alternating intervention of those lipids appears to play a task in a better distribution of oxygen. The oxygen which is mounted by hemoglobin when the crimson cells have passed the lungs, is largely retained as such by the intervention of the sterols till they attain the purpose within the tissues where liberation of oxygen is necessary, this being favored now by the intervention of the fatty acids. The abnormally dark coloration of the blood resulting from its remedy in vitro with polyunsaturated fatty acids has suggested the intervention of such substances in those clinical circumstances wherein related coloration adjustments are noted within the blood as in shock. We'll present our studies in shock beneath. For the moment we are going to solely be aware that in the state of shock experimentally induced by trauma, burns or irradiation, or present in terminally ailing adrenalectomized animals, these animals have not only a excessive fatty acid content material, however that the kind of fatty acids encountered will not be the identical as in regular animals.

Now we have discussed these abnormal fatty acids above. The present differences have been proven by measuring the quantity of oxalic acid that is produced when these fatty acids are submitted to a careful standardized oxidative fission. The oxidative fission of the fatty acids not solely from their whole physique however even from their blood has proven that for normal animals, no oxalic acid might be found, resulting in the assumption that no conjugated fatty acids are present. On the other hand, oxalic acid appeared when fatty acids obtained from animals in shock or BloodVitals monitor from their blood have been damaged down with the analytical method utilized. Of particular significance for the pathogenic function of those fatty acids is the actual fact which we will talk about again beneath, that dying appears to ensue when the conjugation of fatty acids reaches a certain worth, which is approximately the identical whether or not the animal has been traumatized, burned, irradiated or adrenalectomized, BloodVitals monitor and unbiased of the fact that dying occurs in a short while or several days.